These differences are reflected in the unrelated substrate requirements and structures of eukaryotic and prokaryotic luciferases. In bacterial systems it is coupled to the reducing and energy states of the cell, whereas in eukaryotic systems it is dependent upon the availability of ATP. The biochemistry of light production is certainly well-understood today. The biological emission of light has fascinated humankind for mill- nia who is not enchanted by the flashing of fireflies during summer's e- nings? Their collection by children in the area surrounding Baltimore, Maryland in the middle of this century for the purpose of biochemical isolation and ch- acterization of light producing proteins and cofactors may constitute the i- tiation of serious, quantitative studies of this phenomenon.
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